vendor-specific microscopy file format Search Results


confocal laser scanning microscopy spinning disk visitron csu-w1  (VISITRON Inc)
90
VISITRON Inc confocal laser scanning microscopy spinning disk visitron csu-w1
Confocal Laser Scanning Microscopy Spinning Disk Visitron Csu W1, supplied by VISITRON Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vendor-specific+microscopy+file+format/pm37100210-170-8-14?v=VISITRON+Inc
Average 90 stars, based on 1 article reviews
confocal laser scanning microscopy spinning disk visitron csu-w1 - by Bioz Stars, 2026-08
90/100 stars
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nanog  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc nanog
( A ) Fabrication and imaging of the embryoids. Embryoids were fabricated by aggregating human embryonic stem cells (hESCs) in the pyramidal wells and cultured in the 3D gel matrix. Samples collected at multiple time points were immune-stained <t>by</t> <t>fluorescent</t> makers of DAPI, <t>NANOG,</t> GATA3, and T and imaged using confocal microscopy. ( B ) Self-devised image processing pipeline, which contains size adjustment, segmentation, alignment, and standardization. ( C ) Architecture of the transformer-CNN network for image segmentation. ( D ) Examples of experimentally acquired raw images, manually labeled masks and predicted masks of the embryonic tissues, cavities, and single cells by the transformer-CNN network. Green color is for embryonic tissues, yellow is for central cavity, red boundaries indicate single cells, and white dashed curves show the outer boundaries of the embryoids. ( E ) Convergence plots of the cross-entropy (CE) losses and dice losses for tissue segmentation. ( F ) Convergence plots of the CE losses and dice losses for segmentation of single cells.
Nanog, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vendor-specific+microscopy+file+format/pmc12327450-213-37-46?v=Cell+Signaling+Technology+Inc
Average 96 stars, based on 1 article reviews
nanog - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier

Image Search Results


( A ) Fabrication and imaging of the embryoids. Embryoids were fabricated by aggregating human embryonic stem cells (hESCs) in the pyramidal wells and cultured in the 3D gel matrix. Samples collected at multiple time points were immune-stained by fluorescent makers of DAPI, NANOG, GATA3, and T and imaged using confocal microscopy. ( B ) Self-devised image processing pipeline, which contains size adjustment, segmentation, alignment, and standardization. ( C ) Architecture of the transformer-CNN network for image segmentation. ( D ) Examples of experimentally acquired raw images, manually labeled masks and predicted masks of the embryonic tissues, cavities, and single cells by the transformer-CNN network. Green color is for embryonic tissues, yellow is for central cavity, red boundaries indicate single cells, and white dashed curves show the outer boundaries of the embryoids. ( E ) Convergence plots of the cross-entropy (CE) losses and dice losses for tissue segmentation. ( F ) Convergence plots of the CE losses and dice losses for segmentation of single cells.

Journal: Science Advances

Article Title: Deep manifold learning reveals hidden developmental dynamics of a human embryo model

doi: 10.1126/sciadv.adr8901

Figure Lengend Snippet: ( A ) Fabrication and imaging of the embryoids. Embryoids were fabricated by aggregating human embryonic stem cells (hESCs) in the pyramidal wells and cultured in the 3D gel matrix. Samples collected at multiple time points were immune-stained by fluorescent makers of DAPI, NANOG, GATA3, and T and imaged using confocal microscopy. ( B ) Self-devised image processing pipeline, which contains size adjustment, segmentation, alignment, and standardization. ( C ) Architecture of the transformer-CNN network for image segmentation. ( D ) Examples of experimentally acquired raw images, manually labeled masks and predicted masks of the embryonic tissues, cavities, and single cells by the transformer-CNN network. Green color is for embryonic tissues, yellow is for central cavity, red boundaries indicate single cells, and white dashed curves show the outer boundaries of the embryoids. ( E ) Convergence plots of the cross-entropy (CE) losses and dice losses for tissue segmentation. ( F ) Convergence plots of the CE losses and dice losses for segmentation of single cells.

Article Snippet: At multiple culture time points (i.e., 12, 21, 24, 36, 40, 48, 49, 54, and 64 hours), a proportion of embryoids were collected from the well plate and stained with fluorescent markers for DAPI (Thermo Fisher Scientific), NANOG (species: rabbit, dilution: 1:200, catalog no. 4903S, vendor: Cell Signaling Technology), GATA3 (species: mouse, dilution: 1:100, catalog no. MA1-028, vendor: Thermo Fisher Scientific) and T (species: goat, dilution: 1:200, catalog no. PA5-46984 or 81694s, vendor: Thermo Fisher Scientific or Cell Signaling Technology).

Techniques: Imaging, Cell Culture, Staining, Confocal Microscopy, Labeling

( A ) Feature analysis pipeline includes segmentation of embryonic tissues and single cells; identification of GATA3 + , NANOG + , and T + cells; measurement of morphological and fluorescent intensity features; and visualization of the cell distributions. ( B ) Probabilistic distributions of GATA3 + cells, NANOG + cells, and T + cells at five culture time points in the three groups grown from small, intermediate, and large initial cell densities. Color represents three marker types. Large color brightness indicates that there are more marker-positive cells. An embryoid is classified as an asymmetric tissue if its thickness ratio is larger than 1.5. Otherwise, it is regarded as a symmetric tissue. ( C to E ) Group-wise distributions of mean cellular intensities of GATA3, NANOG, and T markers over time. ( F ) Group-wise distributions of the number of GATA3 + , NANOG + , and T + cells. ( G ) Group-wise distributions of tissue thickness surrounding the GATA3 + , NANOG + , and T + cells. ( H ) Group-wise distributions of tissue area over time. ( I ) Group-wise distributions of cell number over time.

Journal: Science Advances

Article Title: Deep manifold learning reveals hidden developmental dynamics of a human embryo model

doi: 10.1126/sciadv.adr8901

Figure Lengend Snippet: ( A ) Feature analysis pipeline includes segmentation of embryonic tissues and single cells; identification of GATA3 + , NANOG + , and T + cells; measurement of morphological and fluorescent intensity features; and visualization of the cell distributions. ( B ) Probabilistic distributions of GATA3 + cells, NANOG + cells, and T + cells at five culture time points in the three groups grown from small, intermediate, and large initial cell densities. Color represents three marker types. Large color brightness indicates that there are more marker-positive cells. An embryoid is classified as an asymmetric tissue if its thickness ratio is larger than 1.5. Otherwise, it is regarded as a symmetric tissue. ( C to E ) Group-wise distributions of mean cellular intensities of GATA3, NANOG, and T markers over time. ( F ) Group-wise distributions of the number of GATA3 + , NANOG + , and T + cells. ( G ) Group-wise distributions of tissue thickness surrounding the GATA3 + , NANOG + , and T + cells. ( H ) Group-wise distributions of tissue area over time. ( I ) Group-wise distributions of cell number over time.

Article Snippet: At multiple culture time points (i.e., 12, 21, 24, 36, 40, 48, 49, 54, and 64 hours), a proportion of embryoids were collected from the well plate and stained with fluorescent markers for DAPI (Thermo Fisher Scientific), NANOG (species: rabbit, dilution: 1:200, catalog no. 4903S, vendor: Cell Signaling Technology), GATA3 (species: mouse, dilution: 1:100, catalog no. MA1-028, vendor: Thermo Fisher Scientific) and T (species: goat, dilution: 1:200, catalog no. PA5-46984 or 81694s, vendor: Thermo Fisher Scientific or Cell Signaling Technology).

Techniques: Marker